›› 2009, Vol. 40 ›› Issue (5): 798-802.doi: 10.3969/j.issn.0529-1356.2009.05.021

• 论著 • 上一篇    下一篇

日本三角涡虫自发荧光组织结构的研究

薛德明 ;陈广文*; 傅山岗 ;任爽; 刘德增   

  1. 河南师范大学生命科学学院,河南 新乡 453007
  • 收稿日期:2008-06-21 修回日期:2008-09-02 出版日期:2009-10-06
  • 通讯作者: 陈广文

Research on histological structure of autofluorescence of EM>Dugesia japonica/EM>

  1. College of Life Science, He′nan Normal University, He′nan Xinxiang 453007, China
  • Received:2008-06-21 Revised:2008-09-02 Online:2009-10-06
  • Contact: CHEN Guang-wen

关键词: 自发荧光, 组织结构, Masson染色, Van Gieson染色, 日本三角涡虫

Abstract: Objective Hermaphroditic planarians possess a very important position in the systematic evolutionary history of animal, as they are capacity of complete regeneration. Hence, the research on histological structure of autofluorescence has been carried out to provide a crucial insight into the developmental and regenerative biology. Methods Part of histological structure of planarian (EM>Dugesia japonica/EM>) was revealed with HE method, Masson method and Van Gieson method. Their autofluorescence was observed with ultraviolet. There were six planarians in each stained group and the autofluorescence group. Results Epidermis, outer epidermis of pharynx, protonephridium, intestine, the photoreceptor cells and longitudinal nerve cords, all radiated blue autofluorescence. The epithelial dissociation side of copulatory bursa radiated yellow autofluorescence, its middle part radiated blue autofluorescence, its fundus side radiated weakly blue autofluorescence. Testis could hardly give off autofluorescence. Pigment cells of eyepot could not give off autofluorescence. Conclusion The research on configuration and autofluorescence of planarian eye may offer help for the study of origin and evolutionary law on eye of invertebrate.

Key words: Autofluorescence, Histological structure, Masson method, Van Gieson method, EM>Dugesia japonica/EM>

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